after purification造句

• Mainly come from geological natural mineral with extremely limited amount and can be removed after purification process
  ，其馀nd主要来自地质本身所含天然矿物，含量极微，经净水处理后即可去除。
• Then after purification , renaturation , cleavage and ultrafiltration centrifugation , igf - 1 and des ( l - 3 ) igf - l protein were obtained
  Westernblot结果表明iptg诱导表达产物可与特异性igf - 1抗体发生反应。
• After purification , the acetylene will be separated by passing through low - pressure water separators ( s101 ) and pressurized by acetylene compressor ( c101 )
  净化后的乙炔气体经低压水分离器（ s101 ）后由乙炔压缩机（ c101 ）增压。
• The believer is to pray in a prescribed manner after purification through ritual ablutions each day at dawn , midday , midafternoon , sunset , and nightfall
  信徒将祈祷以被规定的方式在洗净以后通过礼节洗净液每天在黎明午间midafternoon日落,和黄昏。
• After purification , the acetylene will be pressurized by acetylene compressor ( c101 ) and removed moisture content by acetylene dryer ( a101 ) , and then sent into pipe network for the next process
  净化后的乙炔气体由乙炔压缩机（ c101 ）增压后进入乙炔干燥装置（ a101 ）清除水分，然后送入管网供后续工艺使用。
• In part six , the purification of swnts is discussed and physics - chemistry - connected methods to purify swnts are put forward , the surface shapes of swnts before and after purification using scanning electron microscopy ( sem ) is analyzed and the the purifying effe
  并用扫描电子显微镜分析了纯化前后单壁碳纳米管的表面形态，说明了此方法的纯化效果。用6犯
• After purification , the dna fragment was cloned into pmd - 18t vector directly . the result of identification by pcr method and restriction digestion indicated that the recombinant plasmid had contained a 500bp dna fragment , which demonstrated that we had cloned a part of dna encoding hva1 successfully
  扩增产物经纯化后直接克隆到pmd18 - t载体系统中，经pcr及酶切法鉴定，所得的重组质粒中含有495bp的片段。
• The yeast recombinant expression vector pplc3 . 5k - ndrg2 was constructed , then the ndrg2 protein was expressed in soluable form in pichia . after purification by metal chelate affinity chromatography , we get 6his - ndrg2 fusion which be nature in structure and will be useful to reseach its functions in future
  为研究ndrgz的结构与功能，构建了ndrgz的酵母融合表达质粒，并在比赤酵母中得到可溶形式的表达；利用金属螫合亲合层析纯化出了6his融合的ndrgz蛋白。
• The fine powder flows into the product cyclone collector together with air flow and is expelled from the powder output pipe as product . raymond grinder . in the grinding chamber , since the stuff contains moisture to some extent , raymond grinder the heat generated during grinding makes the moisture evaporated ; since the pipeline joints are not airtight , raymond grinder the external air is sucked in and the circulation air mass increased , in order to enable the grinder to work in negative pressures , raymond grinder the increased air flow is guided to the deduster and sepuentially to the atmosphere after purification . raymond grinder . raymond grinder function tehnical datum
  雷蒙磨粉机-本机适用于粉碎重晶石石灰石陶瓷矿渣等莫氏硬度不大于9 . 3级，湿度在以下的非易燃易爆的矿业冶金化工建材等行业280多余种物料的高细制粉加工如：各种矿石化工原料等，雷蒙磨粉机成品粒度60 425目范围内根据需要任意调节雷蒙磨粉机最细1000目。
• Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease . serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography . the t profile , t stability profile , ph profile , substrate specificity and inhibitor were tested
  该酶分子n -端的氨基酸序列为avidtgveashpef ，通过n -端氨基酸序列设计简并引物，提取被毛孢owvt - 1的总rna ， rt - pcr克隆了该酶的成熟肽编码基因( 1000bp ) ，序列分析表明，丝氨酸含量高达12以上。
• So in this paper , effects of dietary protein levels on the content of protein and amino acid profile in hepatopancreas and ovaries during ovarian maturation are investigated on basis of analyzing protein content and amino acid composition and content in hepatopancreas and ovary of broodstock in nature . influence of dietary protein on concentration of lipovitellin in ovary of broodstock after purification and preparation antibody of lipovitellin from mature ovaries of e . sinensis is evaluated . and nutritional requirement for arginine and lysine , and interaction between protein and vitamin b6 of juvenile e . sinensis are also studied
  华东师范大学2003届博卜学位论文为此，本研究在弄清中华绒鳌蟹自然条件下卵巢发育过程中肝胰腺和卵巢的蛋白质含量及其氨基酸组成的基础上，探讨了饵料蛋白质水平对其卵巢发育过程中肝胰腺和卵巢的蛋白质含量及氨基酸组成的影响;在制备中华绒鳌蟹成熟卵巢卵黄磷蛋白抗体的前提下，研究了蛋白质营养对成熟期卵巢卵黄磷蛋白合成的影响:并对仔蟹期的部分必需氨基酸适宜需要量，蛋白质和维生素b6的相互作用进行了研究。
• After purification and absolv in 8mol / l urea . and then injected rabbits 3 times with 14 days interval . by analysis of indirect elisa , the anti - pea - h3 antibody appear in the rabbit serum induced by the recombinant fusion protein , the highest antibody level was observed during 7 - 14days after the final inoculation
  提取重组质粒并转化大肠杆菌bl21 ( de3 ) ，经iptg诱导阳性菌，对菌体总蛋白进行sds - page电泳分析，结果表明bl21 ( de3 )能有效表达目的蛋白，薄层扫描计算分析，显示表达蛋白带密度积分占总蛋白量的16 . 28 。
• After purification using glutathione sepharose 4b affinity chromatography and digestion with thrombin , the recombinant ntfs were found to be biologically active in the pc 12 cells neurite outgrowth assay . the assays demonstrated that the purified ntfs proteins exhibited normal activity , which is the first step in developing a comprehensive gene therapy for nerve diseases of the giant panda and the crested ibis
  最后，对重组表达蛋白经glutathlonesepharose4bmicrospincolumn亲和纯化后，进行大鼠肾上腺嗜铬瘤细胞（ p2 ）神经营养因子的活性鉴定，发现其能够诱导神经细胞分化产生突触，即具有预期的生物学活性，表明所获表达蛋白为大熊猫nt
• The e2 gene was amplified by rt - pcr , then examined the fragment by electrophoresis . after purification and insertion into pucm - t vecter , the recombinant plasmid pbne2pi and pbne2pii were obtained . then they were transfected e . coli jm109 and screened positive clones by blue or white plaques . the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis
  通过rt - pcr扩增e _ 2基因，电泳测定扩增片段的大小，经纯化后，连接于pucm - t载体，获得重组质粒pbne2p 、 pbne2p ，转化e ? colijm109 ，经蓝白斑筛选，挑取阳性克隆，提取质粒，直接电泳鉴定和酶切鉴定。
• At the same time an apparatus of zone melting is designed , the method of zone melting is used to purify the purified product of recrystalizing . after purification , the homogeneity and stability of the purified product of antioxidant d and bht are examined by high performance liquid chromatography ( hplc )
  实验研究结果：防老剂d重结晶提纯工艺为：每次取10g工业品防老剂d ，经过碱洗后，去离子水洗涤至中性，溶于100ml乙醇中，同时用粉状活性炭( 1g次)脱色、过滤、母液静置2h后，再放入冰箱结晶2h 。